Katarina Petra van Midden, PhD student
I am a PhD student in Biochemistry and am fascinated by cell death and pathways that regulate it. That is why the main focus of my research is studying the role of proteases in the process of cell death in unicellular organisms. The model organism for my research is the unicellular alga Chlamydomonas reinhradtii. I aim to elucidate the role of proteases in this process using different approaches – enzyme assays, protein purification, microscopy and localization of target proteases and identification of protease degradome.
Tanja Peric, MSc student
I am a master degree student of Biochemistry. Currently, I’m working on determining the effect of structural differences in metacaspases on the function and activity of these proteins. For this purpose, I am using wild type and modified metacaspases from unicellular algae Chlamydomonas reinhardtii and Guillardia theta. I am a master degree student of Biochemistry. Currently, I’m working on determining the effect of structural differences in metacaspases on the function and activity of th
Lana Vogrinec, MSc student
My main focus in the lab is functional characterization of the orthocaspase SyOC from the model cyanobacterium Synechocystis sp. PCC 6803. Methodology includes phenotypic analysis of the wild type and mutant strain, which lacks the gene for SyOC orthocaspase, as well as biochemical characterisation of the isolated protein SyOC. I am a master degree student of Biochemistry. Currently, I’m working on determining the effect of structural differences in metacaspases on the function and activity of th
Doroteja Armič, MSc student
I am a master degree student in Biochemistry. My focus of research is the vacuolar processing enzyme from a unicellular alga Chlamydomonas reinhardtii. The vacuolar processing enzyme is involved in the initiation of apoptosis in plants and I find it very fascinating. I am currently working on expressing this protein in bacteria and my goal is its biochemical characterization. apoptosis in plants and I find it very fascinating. I am currently working on expressing this protein in bacteria and my goal is its biochemical characterization.
Anže Jenko, MSc student
I am a master’s degree student in Biochemistry. The purpose of my research is to express and biochemically characterize structurally unusual type II metacaspase found in cotton plant Gossypium raimondii, a close relative of economically important upland cotton plant G. hirsutum. Metacaspases are putatively involved in numerous cellular processes and pathways, including regulated cell death which especially fascinates me. I aim to elucidate the role of this structural peculiarity in metacaspase and its effect on enzymatic activity and function.
Tadej Medved, MSc student
The aim of my research encompasses the dihydroorotate dehydrogenase enzyme of the malaria parasite Plasmodium falciparum. Catalysing the rate-limiting step of pyrimidine biosynthesis in P. falciparum, this enzyme is a promising target for the development of small-molecule antimalarials. I am currently working on expressing a recombinant version of this enzyme and crystallising it in complex with pyrazolidinone inhibitors in order to elucidate the structural basis of their inhibition.
Michelle Oletič, MSc student
I am a master’s student in biochemistry. Currently, my main focus is on the genomic toxin-antitoxin system of Microcystis aeruginosa PCC 7806, which is located next to the orthocaspase transcript MaOC1, which is responsible for the degradation of the antitoxin. This means that it could regulate the level of free toxin in the cell. I am currently working on finding out where these cleavage sites might be and how that influences the stability of the antitoxin or the toxin-antitoxin complex.
Vivian Nemanič, BSc student
I am an undergraduate student in biochemistry. My focus in the lab is toxin-antitoxin systems, which are also found in cyanobacteria. They have several functions, including programmed cell death, plasmid stability, and growth inhibition. In Microcystis aeruginosa PCC 7806, in addition to the toxin-antitoxin system, there is a transcript of the orthocaspase MaOC1, which is responsible for degrading the antitoxin, and my main focus was to find its cleavage sites.