Metacaspases

Metacaspases represent the largest subgroup in the superfamily of caspases proteins and can further be devided into four classes, depending on the presence as well as the location of the p10 domain.

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Domain architecture and properites of caspases and metacaspases.

 

Excluding metacaspase-like proteases, which lack the p10 domain, metacaspases of types I, II and III all depend on the presence of calcium for their activity. We have recently shown [Klemencic and Funk, 2018] that it is the aspartic acid residues in the p10 domain that are required for proteolytic activity of these enzymes.

Further experiments are directed in more detailed characterisation of each of the three recombinantly prepared metacaspase types as well as their biochemical characterisation in vivo.